TILLING platform

Over the last two decades, knowledge about plant growth, development and the molecular compositions of plant organs has increased tremendously. The genes that control the function of the biological mechanisms involved are in many cases identified and well characterized. Unfortunately, development of technologies to manipulate such genes in crop genomes did not match that progress.

To bridge the gap between structural genomics and functional genomics in crop plants, the Crop Genomics Group invested on the development of two complementary reverse genetics tools, TILLING and ECOTILLING. TILLING is based on production of EMS-mutagenised plant collections and rapid systematic identification of mutations in target sequences. ECOTILLING is based on the analysis of germplasm collections to identify natural alleles of a given gene.

Four distinct research areas were at the core of our project:

Today, the TILLING platform has developed more than 14 mutant collections in 8 crop species and 5 core collections in 4 species.

To manage and integrate the data from both the phenotype recordings and TILLING platform, we implemented the database UTILLdb -. Two main types of data are accessible, sequences of tilled genes and the corresponding alleles and when available, the morphological phenotypes of the mutants.

The TILLING platform is involved in more than 30 projects and more than 50 collaborations.
Since 2007, we have published 17 publications with an average IF=11,5. Three of them have been published in Nature, Science and Nature Genetics.

News

- Most recent collections

- Cucumber
- Watermelon

- Latest publications

A conserved molecular basis for photoperiod adaptation in two temperate legumes, PNAS, 2012.
A role for an endosperm-localized subtilase in the control of seed size in legumes, New Phytol, 2012.
The TERMINATING FLOWER gene of tomato defines a novel phase of flowering, Nature Genetics, 2012.

- Going to TILLING via NGS

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