-

 

-

Brachypodium distachyon

For a decade, Brachypodium distachyon (Brachypodium) is a model plant for temperate grasses and cereals such as wheat, barley and oat. Its small size and easy cultivation make it easy to use in laboratories without any expensive cost and easy to handle for several topics such as root development, biomass production and cell wall biosynthesis for instance. Interestingly, Brachypodium has one of the smallest grass genome (272 Mb) which is sequenced since early 2010.

To elaborate a first generation of tools in Brachypodium that will allow a rapid access to reverse genetics tools, we developed in collaboration with R. Sibout (INRA-IJPB, Fance) a Brachypodium distachyon in silico forward and reverse genetics tool. Within the French INRA program ALICE, focused on the ligno-cellulose improvement for biofuel dedicated plants, a reference NaN3-mutant population was created from Brachypodium distachyon Cv Bd21-3 under controlled conditions and phenotypic data based on visual characterization of M2 plants was recorded from young seedling to fruit maturation stages.

Another available tool developed on the TILLING platform is the exploitation of natural variation in Brachypodium, using an EcoTILLING population, corresponding to one core collection developed in collaboration with INRA-IJPB who managed both plant production as well as seed stock.

 

Key features on the Brachypodium Bd21-3 TILLING platform:

- 5530 M2 families
- More than 19 genes screened
- Mutation frequency: 1/ 354Kb
- Both phenotypical and sequence data are available in the URGV TILLING database -
- Bd21-3 mutant collection was done in collaboration with INRA-IJPB (Versailles, France) who managed both plant production as well as seed stock.
- Contact: Marion Dalmais, dalmais@evry.inra.fr, +33 1 60 87 45 12